Ultrasensitive Detection of Chemical Substances
Abstract
Vital to any tight binding detection system is the strength of the bond joining the signal to the target molecule. For antibody/antigen systems, this requires a low dissociation constant and a correspondingly high intrinsic affinity constant. We have calculated an affinity constant for our AChE/DFP specific Ab. For the past year we have used passive binding to create a surface of AChE or another antigen on a polystyrene support. For an efficient ultrasensitive detection system, it is important that the trapping agent (AChE) can be bound to the solid support at high density. In the past months we have discovered that Rivanol, an agent used normally to purify antibody, significantly increases the binding of AChE and selected other proteins to polystyrene solid supports. While our investigation of the mechanism of how Rivanol increases binding which is pH dependent. Illustrated in the four graphs that follow is the pH and concentration effect of Rivanol on the binding of AChE/DFP conjugate.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 28, 1985
- Accession Number
- ADA152276
Entities
People
- R. G. Dean
Organizations
- University of New Mexico