Studies of the Biology of Phleboviruses in Sandflies.
Abstract
Laboratory colonies of three new sand fly species (Phlebotomus perniciosus, P. argentipes and P. Martini) were established. In addition, a continuous culture of Phlebotomus papatasi cells was also started. Attempts to clone the latter cell line have not been successful. The growth of six representative phleboviruses (Toscana, Belterra, Naples, Arbia, Punta Toro and Pacui) was studied in sand flies following inoculation and ingestion. The pattern after inoculation was similar; maximum virus titers were reached on the third or fourth day after infection and then seemed to stabilize at this level. In contrast, sand flies appeared to be fairly refractory to oral infection. An attempt was made to develop a sand fly colony, chronically infected with a phlebovirus. Three virus-vector combinations were tried (Pacui virus and Lutzomyia longipalpis, Karimabad virus and P. papatasi, and Toscana virus with P. perniciosus). The Toscana - P. perniciosus combination gave the most interesting results. Transovarial infection rates in the F1 and F2 generations of these parenterally infected parent females was 30 and 41% respectively. Filial infection rates among F2 generation adults from individual transovarially infected F1 parents varied from 5 to 100%.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1984
- Accession Number
- ADA152918
Entities
People
- R. B. Tesh
Organizations
- Yale University