Bactericidal Capability and Respiratory Burst Characteristics of the Murine Macrophage Cell Line RAW264.7.

Abstract

The murine macrophage RAW264.7 cell line was used to establish assays of phagocyte functional status as part of an in vitro toxicity testing program. The variables included antimicrobial activity (toward S. epidermidis) and the respiratory burst (oxygen consumption, superoxide anion radical and hydrogen peroxide release) in response to 12-0-tetradecanoyl-13-phorbol acetate (TPA) and opsonized zymosan. The cells had potent antimicrobial activity. The magnitude of the respiratory burst in oxygen consumption was highly dependent on culture conditions. Additional keywords: cell culture bactericidial assays; bactericidal potency; Stanford cells.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1985
Accession Number
ADA152985

Entities

People

  • B. D. Schwartz
  • E. L. Mcgown
  • J. R. Hillman
  • M. G. Rusnak
  • S. F. Orencole

Organizations

  • Letterman Army Hospital

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anti-Infective Agents
  • Biological Sciences
  • Blood
  • Cell Line
  • Cells
  • Cellular Structures
  • Chemical Kinetics
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Hydrogen
  • Macrophages
  • Medical Personnel
  • Military Research
  • Phagocytes
  • Respiration
  • Rocket Oxidizers

Fields of Study

  • Biology

Readers

  • Immunology
  • Immunology and Pathology
  • Solar Physics