Rapid Rate-Kinetic Turbidometric Assay for Quantitation of Antiviral Complement-Fixing Antibodies.
Abstract
A rapid rate-kinetic turbidometric assay for the quantitation of viral complement fixing antibodies has been developed, using adenovirus as a model. The procedure is based on the turbidometric quantitation of intact sheep erythrocytes and measures the rate of hemolysis (change in absorbance per min at 640 nm) at maximum velocity occurring in the presence of residual complement not fixed by the antigen-antibody reaction. Reagents were standardized and assays performed using a microprocessor-controlled spectrophotometer with thermoregulated cell compartment and kinetic assay capability. Eleven sera were assayed for complement fixing antibodies both by the conventional microtiter technique and by the rapid turbidometric described here. Good correlation (r = 0.88) between the two procedures was obtained. Unlike the conventional complement fixation test, in the rate-kinetic turbidometric complement fixation test, it was found that variation in complement and antigen concentration could be tolerated, end-pointers titers were objectively quantitated, and results could be obtained within one hour. The technique may be adaptable to large-scale automation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1985
- Accession Number
- ADA153644
Entities
People
- R. E. Fulton
- V. L. Dininno
Organizations
- Defence Research and Development Canada