Studies on the Immunochemical Techniques for Detection of Selected Fungal and Dinoflagellate Toxins

Abstract

The goal of this contract is to develop a method for the production of antibodies against several selected mycotoxins and dinoflagellate phytotoxins and subsequently to develop a radioimmunoassay (RIA) or an enzyme-linked immunosorbent assay (ELISA) for toxin determination. During the first nine months, effective methods for the preparation of hemisuccinate (HS) and hemiglutarate (HG) of T-2 toxin, HS of diacetoxyscirpenol (DAS), and 0- carboxymethyl oxime (0-CM) of vomitoxin (deoxynivalenol, VT) were developed. Methods for the preparation of reduced saxitoxin (STX) as well as HS of reduced saxitoxin (STXOL) were also established. These derivatives were chemically characterized and were conjugated to bovine serum albumin (BSA) for subsequent immunization. They have also been conjugated to horseradish peroxidase (HRP) for direct enzyme linked immunosorbent assay (ELISA) and to polylysine and hemocyanin for indirect ELISA. The toxicy of T-2 HS (LD50) was determined in mice. For antibody production, 26 rabbits were immunized with various toxin-BSA conjugates.

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Document Details

Document Type
Technical Report
Publication Date
Aug 15, 1982
Accession Number
ADA153832

Entities

People

  • F. S. Chu

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Acetic Acid
  • Albumins
  • Alcohols
  • Anhydrides
  • Antibodies
  • Biomedical Research
  • Fungi
  • Gels
  • Imines
  • Immune Serums
  • Medical Personnel
  • Monitoring
  • Phase
  • Proteins
  • Silica Gels
  • Silicon Dioxide

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology
  • Molecular and Cellular Biochemistry
  • Mycotoxin ecology in Amazonian ecosystems.