Tissue Culture and in Vivo Modelling of Corneal Opacification and Ocular Injuries Induced by Millimeter Waves.
Abstract
The eventual aims of these experiments are to use intact corneas incubated in vitro and corneas of rats in vivo to high energy pulsed millimeter waves to study the development of corneal damage. Such experiments are expected to establish conditions for corneal damage and to eluciodate the mechanisms by which the damage occurs. If intact corneal explants are cultured in tissue culture dishes in medium 35.5 C continuing outgrowth of the epithelial cells occurrs from the edge of the explants during a period of at least two weeks. Exposure for shorter periods of such cultured corneas to elevated temperatures and vitamin A followed by fixation for light microscopy (SEM) revealed progressive cellular damage as the temperature increased and some vitamin uptake which is being studied further, buy biochemical techniques. Originator supplied key words: Rabbit/rat eye cornea, Millimeter wave irradiation, Pulsed and continous waves, Tissue culture medium, Elevated temperature, and Vitamin E prevention.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1982
- Accession Number
- ADA153854
Entities
People
- J. R. Trevithick
Organizations
- Western University