Physical Chemical State of Water in Living Cells.
Abstract
Overall Research Program (Specific work tasks) outlined in the proposal include: Physical properties of water in tissues, cells and subcellular organelles; Physical properties of water in the brine shrimp Artemia; Effects of hemorrhagic shock on the physical properties of tissue water. Relaxation times and diffusion coefficients for water protons, hydrogen and isotopes of water were determined. The progress 1983-84 pertains to: Mechanisms of NMR Relaxation: Hypothesis of Proteins Dynamics and Hemorrhagic Shock. A Biophysical Study of Cellular Water and Ions in Excitable Cells - Functional Role of Ion-Water-Protein Interactions in Excitable Cells is summarized. Scientific findings include: (1) Effects of the ionic environment on the gating properties of the excitable ionic channels; (a) Effect of internal Cs(+) ions on the early and late conductance in squid axon; (b) The effect of internal sodium ions on the action potential and reversal potential in squid axons, (2) Structure and function of the membrane proteins and the submembrane (cortical) protein network; (a) Structure of the squid axon membrane as seen after freeze-fracture; (b) Possible role of cytoplasmic microtubule structure in the excitation properties of nerve axon; (3) Ionic mechanisms involved in the regulation of the resting potential; (a) Effects of ionic concentration on the resting potential in squid axons: Evidence supporting a modification of the constant field equation; (b) Differentiation between the excitable sodium channel and the major pathway for the resting sodium current; (4) Intracellular transport of Na(+) ions inside the nerve cell.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1985
- Accession Number
- ADA154566
Entities
People
- C. F. Hazlewood
- David Chang
- R. L. Vick
Organizations
- Baylor College of Medicine