Antigenic Characterization of Locally Prevalent Rabies Strains Using Monoclonal Antibodies,
Abstract
The production of monoclonal antibodies has been made feasible by hybridoma technology. In the case of rabies, it was possible to obtain hybridomas that secrete antibodies to the nucleocapsid determinants of the virus and others that secrete antibodies to the glycoprotein determinants. The production of such antibodies provided the opportunity to study and antigenically analyze the street rabies virus strains prevalent in Egypt. In this communication we give the results of antigenic characterization of 19 rabies strains using monoclonal antibodies to the nucleocapsid fraction of the rabies virus and one monoclonal to the Mokola rabies-related virus. The first 2 strains were from human rabies cases. The next 9 strains were isolated from rabid dogs, strains 12 and 13 from cats, strain 14 from a donkey, strain 15 from a sheep, strains 16-18 from gerbils and strain 19 is the Challenge Virus Standard (CVS) maintained by mouse passages in our laboratory since 1972. Results indicate the nucleocapsid identity of the human and domestic animal strains tested.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 05, 1985
- Accession Number
- ADA154908
Entities
People
- B. A. M. Botros
- D. M. Robinson
- J. M. Linn
Organizations
- Naval Medical Research Unit Three