Specificity of Monoclonal Antibodies Reactive with Fusobacterium nucleatum: Effect of Formalin Fixation,

Abstract

Monoclonal antibody specific for Fusobacterium nucleatum was reacted with untreated and formalin fixed F. nucleatum cells by an enzyme-linked immunosorbent assay (ELISA) and by indirect immunofluorescence. Treatment of bacterial cells with formalin destroyed the antigenic determinant responsible for reactivity with this monoclonal antibody in both assays. Formalin fixation had no effect on hemagglutination activity (HA) of F. nucleatum cells or reactivity with polyvalent rabbit antiserum by double diffusion in agar. Scanning electron microscopy demonstrated that formalin fixation did not affect binding of F. nucleatum cells to microtiter plates. When developing monoclonal antibodies that will be used as diagnostic reagents, the antigen form utilized for immunization should be identical to the antigen form which will eventually be used in the diagnostic assay.

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Document Details

Document Type
Technical Report
Publication Date
Jun 03, 1985
Accession Number
ADA156171

Entities

People

  • J. W. Vincent
  • W. A. Falkler Jr.
  • W. A. Gray
  • W. C. Cornett

Organizations

  • United States Army Institute of Surgical Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Antibodies
  • Blood
  • Blood Cells
  • Cell Line
  • Cells
  • Classification
  • Electron Microscopy
  • Electrons
  • Fusobacteria
  • Laboratory Animals
  • Materials
  • Microscopy
  • Proteins
  • Scanning
  • Scanning Electron Microscopy
  • Security

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular Genetics
  • Toxicology/Environmental Toxicology

Technology Areas

  • Microelectronics