Study of Toxic and Antigenic Structures of Botulinum Neurotoxins.

Abstract

Amino acid compositions of botulinum neurotoxin (NT) types A, B, E and F were determined. The heavy and light chains of types A, B and E NT were separated, purified, and analyzed for amino acid compositions and partial N- terminal sequence. Selective modification of tyr, his and arg residues and also carboxyl and amino groups of types A and E NT demonstrated the role of these amino acid residues in toxicity and antigenicity. Type E NT completely detoxified following modification of his, tyr or amino groups has potential as a toxoid. Effects of types A and B NT on rat neuromuscular junctions appear non- identical. Additional keywords: immunology; bacterial toxins.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1985
Accession Number
ADA156642

Entities

People

  • B. R. Dasgupta

Organizations

  • University of Wisconsin–Madison

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Amines
  • Amino Acids
  • Biomedical Research
  • Botulism
  • Chemistry
  • Contracts
  • Demographic Cohorts
  • Detoxification
  • Gel Electrophoresis
  • Histidine
  • Ion Exchange
  • Lethality
  • Peripheral Nervous System
  • Therapy
  • Toxicity
  • Vaccines

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry