Laboratory Evaluation of Adenylate Energy Charge as a Test for Stress in Mytilus edulis and Nephtys incisa Treated with Dredged Material.
Abstract
Changes in adenine nucleotide metabolism were evaluated as indices of stress in the marine bivalve Mytilus edulis and the polychaete Nephtys incisa when treated with highly contaminated dredged material under laboratory conditions. Anesthetization of N. incisa is necessary to maximize the adenosine triphosphate (ATP) concentration and the adenylate energy charge (AEC) by eliminating all swimming activity and facilitating handling upon their removal from sediment. Extraction of adenine nucleotides from N. incisa required the addition of ethylenediaminetetraacetic acid to perchloric acid to obtain consistency high recover efficiencies of 96 + or - 0.3 percent for ATP. Perchloric acid alone gave consistent recovery efficiencies of 92 + or - 0.5 percent of ATP from adductor muscle tissue of M. edulis. Reproductibility within and between tests is exceptionally good for both M. edulis and N. incisa. Both M. edulis and N. incisa are excellent species with which AEC can be used to accurately assess their metabolic state and health condition when exposed to sublethal environmental perturbations.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1985
- Accession Number
- ADA157374
Entities
People
- C. Pesch
- Dolores Black
- G. E. Zaroogian
- P. Schauer