Immunotechnology.
Abstract
The control of T and B lymphocyte responses is a major goal of immunobiology. In this study we have examined the control of B cell responses in vitro by several lymphokines including IL-1, IL-2 and gamma interferon. This analysis provides information on the potential to manipulate B cells in culture for their production of desired antibodies, as may be needed prior to hybridoma production and also provides information on the mechanism of regulation of lymphocyte responses. In the present study we have shown that IL-1 and more importantly gamma interferon are involved in the regulation of B cell responses. The role of gamma interferon in T cell-replacing factor (TRF) activity for antigen-specific plaque-forming cell (PFC) responses in vitro was studied using antibodies to murine gamma interferon. TRF activity was present in supernatants (Sn) of Con A- or mixed leukocyte reaction-stimulated murine spleen cells as well as in an IL-2-rich fraction of phytohemagglutinin-simulated murine human peripheral blood lymphocyte Sn and in the Sn of the Gibbon T lymphoma MLA-144. The human TRF was highly active with cells from nu/nu mice and normal mice but not with cells from animals with the xid immunologic defect, similiar to the activity of murine TRF.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 31, 1984
- Accession Number
- ADA157935
Entities
People
- P. Lake
Organizations
- Georgetown University