Development of Vaccines to the Mycotoxin T-2
Abstract
An ELIAS System to detect and titer antibody to Mycotoxin T-2 was standardized. A new rapid simple method of making T-2 hemisuccinate (T-2-HS) was developed. The merit of the new method over the existing one accounts for its simple, less time-consuming procedure, which can be carried out at room temperature, with as high a yield of 66%. T-2-HS so developed was conjugated to derivatised sepharope-4B beads to make a column to affinity purify the antibody to Mycotoxin T-2. Polyclonal antisera to T-2 toxin was produced by immunizing rats and mice with T-2-HS coupled to KLH, goat antimouse IgG and monoclonal antimouse IgD. Affinity purified antibody to T-2 was employed in immunizing mice for the development of anti-idiotypic antibodies. Initially syngeneic immunization were carried out in Balb/c mice. Protective role anti-T-2 antibodies upon passive transfer, was examined. Both monoclonal and polyclonal antibodies. to T-2, were found to neutralize T-2 toxin in vitro. Upon passive transfer to skin intradermally, inhibition of skin necrotization effect due to T-2 toxin was demonstrated, suggesting, role of an effective antidote. Protective immunity to in vivo exposure to T-2 toxin, upon active immunization with T-2-HS was also examined. Skin necrotization effect of T-2 toxin was completely inhibited in immune rats. However, the in vivo effect of Oral T-2 toxin, as measured by leucopenia in actively immunized rats, was not dramatically significant.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 15, 1985
- Accession Number
- ADA158544
Entities
People
- Vijaya Manohar