Early Phase Interactions of Toluene with Membranes: A Structural and Functional Evaluation.
Abstract
The principal objective of the research proposed was to define the subcellular site(s) or target(s) of action of the aromatic hydrocarbon toluene. Confirmed target sites were then to be investigated in detail to elucidate possible mechanisms of toluene action in pertubating membrane structure that might be related to either an enhancement or loss in membrane functions. Under this problem, several test systems developed in our laboratory for toxicological evaluation of target sites of membrane active substances were employed. The basic approach was to subject each tissue to a graded series of toluene concentrations for varying periods of time after which the material was prepared for electron microscopy under conditions developed to yield accurate and reproducible evaluations. Comparisons were to identical tissues treated in a similar fashion in the absence of toluene. Gross and subtle morphological changes were noted indicative of an activity target using the following three test systems: Primary Rat Hepatocytes in Culture, Cultured BHK, KB and L Cells, Outer Cap Cells of the Maize Root Tip.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 24, 1984
- Accession Number
- ADA159065
Entities
People
- D. J. Morre
Organizations
- Purdue University