Histologic Changes Caused by Application of Lewisite Analogs to Mouse Skin and Human Skin Xenografts
Abstract
Phenyldichloroarsine (PDA), a vesicating analog of lewisite, was applied in an ethanol carrier to human skin xenografts on nude mice and directly to the ungrafted nude mouse skin. Controls areas received ethanol. Under light microscopy, we observed the following changes in PDA-treated human skin grafts: 1) epidermal cellular nuclear degeneration (apparent by 4 hr with increasing severity through 48 hr); 2) loss of epidermal cytoplasmic basophilia (apparent by 4 hr, maximum within 12 hr); 3) epidermal cytoplasmic vacuolization (vacuoles appeared within 4 hr and increased in size through 24 hr); 4) cleft formation within the basement membrane zone (apparent by 12 hr, increasing in severity through 24 hr); 5) inflammation (polymorphonuclear leukocyte (PMN) infiltration) , apparent by 4 hr and increasing through 48 hr. The following additional lewisite analogs were applied in ethanol carriers to nude mouse skin: phenylarsine oxide, phenydiiodoarsine, (trans) chlorovinylarsine oxide and (trans) chlorovinyldiiodide. The lesions caused by these analogs were reproducible and histologically indistinguishable from that cause by exposure to PDA. The identities of the molecular lesions and the locations of the arsenical- sensitive sites are unknown.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1985
- Accession Number
- ADA159554
Entities
People
- Cecilio R. Damlao
- Evelyn L. Mcgown
- Kenneth E. Black
- Richard J. O'connor
- Theodore Van Ravenswaay
Organizations
- Letterman Army Hospital