Ultrasensitive Detection of Chemical Substances
Abstract
In this reporting period we have continued to characterize monoclonal antibodies against ADhE-DFP. We have focused attention three monoclonals of interest. These are the V-16 antibody described in our previous report and two additional antibodies designated V-18 and D11B. The V-16 antibody demonstrates higher activity against AChE-DFP conjugate than against AChE alone. The V-18 and D11B antibodies showed higher activity against AChE than against the conjugate. In addition, the D11B antibody blocks AChE enzymatic activity in the Elman assay. Further study showed that these antibodies exhibit a different response to AChE-DFP conjugates on the basis that the AChE ages or loses and isopropoxy residue. The resulting phosphate diester has a charge, at normal pH, on oxygen.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 31, 1985
- Accession Number
- ADA159996
Entities
People
- Roger G. Dean
Organizations
- University of New Mexico