Identification and Quantification of the Water-Soluble Components of JP-4 and a Determination of Their Biological Effects upon Selected Freshwater Organisms.
Abstract
This final technical report includes a brief summary of research performed, results obtained, graduate students supported and theses written. One paper published was of a technical nature and describes the use of reversephase C-18 minicolumns for concentrating water soluble hydrocarbons derived from JP-4 jet fuel. Another technical paper using the same technique as the first was used to concentrate water soluble hydrocarbons produced by running an outboard motor in water. Analytical methods used for hydrocarbon separation and identification was GC, GC/MS and HPLC. The toxicity of toluene on the fathead minnow was the basis of three papers and a Ph.D. thesis. It was found that the embryo was as sensitive to toluene than was the protolarvae or adult fish. This was determined using 96-hr LC50 tests. An MS thesis was written on the effects of toluene on gill structure in the fathead minnow adult. Little effect of toluene on gill structure was noted. A comparative study on the effects of administration of benzene, toluene and xylene isomeres on their in vitro metabolism and various drug metabolizing enzymes in rat liver, and the covalent binding of toluene to rat liver microsomes has resulted in one Ph.D. thesis and the preparation of three manuscripts for publication. Originator supplied keywords include: Enzyme induction; Cytochrome P-450; Mixed function oxidase; Conjugation; Cytochrome c reductase; Microsomes; Bluegill sunfish.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1985
- Accession Number
- ADA160271
Entities
People
- J. D. Brammer
- R. L. Puyear
Organizations
- North Dakota State University