DNA and Protein Studies of HLA Class II Molecules: Their Relationship to T Cell Recognition,

Abstract

The HLA-D region, encoding class II antigens, was first defined based on studies of mixed leukocyte culture (MLC) (Bain, et al. 1964; Bach and Hirschhorn, 1964). In our studies with colleagues (Amos and Bach, 1968; Bach et al. 1969), we were able to suggest that a genetic region encoding molecules that stimulate proliferation in MLC may be linked to, but separate from, the HLA-A and -B loci, i.e. a region such as HLA-D; definitive studies in this regard (Yunis and Amos, 1971) established the existence of the HLA-D region. Great advances have been made in our understanding of this region in recent years utilizing both the tools of molecular genetics and studies of proteins encoded by HLA-D genes. A critical bridge that has not yet been completed concerns the relationship of class II genes and protein products to determinants recognized by T lymphocytes, i.e. the cells responding in MLC. Although the emphasis in this review will be on studies of proteins and of DNA, an introduction to this article also includes our current state of knowledge regarding definition of class II products with T lymphocytes. The inclusion in this volume of several articles on the same topic allows a measure of comfort with such an approach.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1985
Accession Number
ADA160704

Entities

People

  • C. A. Dahl
  • J. M. Curtsinger
  • J. S. Cairns
  • M. Segall
  • S. Freeman

Organizations

  • University of Minnesota

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antibodies
  • Cell Line
  • Cells
  • Coding
  • Demography
  • Genes
  • Genetics
  • Leukocytes
  • Liquid Chromatography
  • Lymphocytes
  • Molecular Genetics
  • Molecules
  • Proteins
  • Recognition
  • T Lymphocytes
  • Two Dimensional

Fields of Study

  • Biology
  • Medicine

Readers

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  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech