Indirect Immunofluorescence, Serum Neutralization, and Viremia Responses of Thesus Monkeys (Macaca Mulatta) to Machupo Virus,
Abstract
Although indirect immunofluorescent antibody tests (IFAT) have been developed for several arenaviruses, none has been applied to the rhesus monkey model for Bolivian hemorrhagic fever (caused by the arenavirus Machupo). We infected eight rhesus monkeys with Machupo virus and bled them weekly postinoculation (PI) for determination of viremia and for serum antibody by IFAT and serum neutralization (SN) testing. Viremia peaked 14 days PI, when two of eight animals had low IFAT titers. At 21 days PI, the six surviving monkeys had elevated IFAT titers and diminished viremias. SN titers were not observed until 28 days PI, when three of four survivors had low titers. Results of the IFAT were available more rapidly than the SN, and detected increased serum antibody titers earlier than the SN.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 22, 1985
- Accession Number
- ADA161868
Entities
People
- E. Gonder
- G. Eddy
Organizations
- United States Army Medical Research Institute of Infectious Diseases