Japanese Encephalitis Virus Immunoglobulin M Antibodies in Porcine Sera
Abstract
A solid-phase enzyme-linked immunosorbent assay (ELISA) was developed for detection of porcine immunoglobulin (Ig)M antibodies to Japanese encephalitis virus (JEV). Antibodies in sera were captured onto the solid phase of Microtiter plates sensitized with mouse monoclonal antibodies to porcine u heavy chain. Virus antigen binding to the lawn of IgM as quantitated by subsequent binding of peroxidase-labeled human hyperimmune anti-JEV IgG, which, in the final step, catalyzed a substrate color change.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1985
- Accession Number
- ADA162267
Entities
People
- D. S. Burke
- M. R. Elwell
- Markapol Tingpalapong
- P. S. Paul
- R. A. Van Deusen
Organizations
- Armed Forces Research Institute of Medical Sciences