Bimane Derivatives as Fluorescent Probes for Biological Macromolecules.
Abstract
Nine years ago, we discovered and developed convenient syntheses for a new class of heterocyclic molecules, the bimanes or 1,5-diazabicyclo(3.3.0) octadienediones. In the course of preparing the bromo derivatives, we found fortuitously that proteins were fluorescently labeled by bromobimanes. We soon established that bromobimanes reacted preferentially with thiols, and had demonstrated the usefulness of such labeling for both small and large molecules in biological systems. Many applications of the agents have been reported. The primary aims of our research on the bimanes are divided into the following areas: (a) synthesis (b) reactivity (c) photophysics (d) application to macromolecules. Although we have a number of new results, it seems pertinent to base this report on a new summary of the application of bimane labeling to thiols.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1985
- Accession Number
- ADA162725
Entities
People
- Edward M. Kosower
Organizations
- Tel Aviv University