ELISA (Enzyme-linked Immunosorbent Assay) to Detect Humoral Antibodies Specific for Clostridium botulinum Type A Neurotoxin

Abstract

The enzyme-linked immunosorbent assay (ELISA) was modified for the rapid detection of human humoral antibodies (IgG) that are specific for Clostridium botulinum type a toxoid. The ELISA was useful for the rapid evaluation of immunization results and for distinguishing (95%) immune from nonimmune individuals. ELISA values, when compared with standard in vivo toxin neutralization test values, paralleled the anamnestic response to booster immunization in 25 to 25 individuals.

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Document Details

Document Type
Technical Report
Publication Date
Nov 19, 1985
Accession Number
ADA163060

Entities

People

  • George E. Lewis Jr.
  • Glen A. Higbee
  • Joseph F. Metzger
  • Salvatore S. Kulinski

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Assays
  • Bioassay
  • Biomedical Research
  • Botulism
  • Clostridium
  • Computer Science
  • Confidence Limits
  • Detection
  • Diseases And Disorders
  • Health
  • Health Services
  • Immunity
  • Immunization
  • Laboratory Animals
  • Lethal Dosage
  • Therapy
  • Toxicity

Fields of Study

  • Biology

Readers

  • Immunology
  • Microbial Pathology