Acta Genetica Sinica (Selected Articles),
Abstract
1. Genetic analyses of polymeric genes for maltose fermentation in three species of Saccharomyces were undertaken using S. globosus and S. chevalieri as recessive strains. The experimental results were as follows: 1) S. cerevisiae strain 2.982 contains two polymeric genes for fast maltose fermentation, MAL I and MAL II, each on different chromosomes; 2) S. carlbergensis strain 2.500 contains only one gene, MAL 6; 3) Among the progeny of a hybrid of S. uvarum strain 2.346 with S. chevalieri, S. uvarum has at least one gene, MAL I; 4) S. globosus 2.1161 contains genes MAL I, MAL II, and MAL 6 genes. 2. Tn233 (CH) is a transposon isolated from a resistant Shigella strain in our clinic; its physical map reveals a structure basically similar to transposon Tn21. Plasmid pBR322::Tn233 (CH) DNA with transposon Tn233 (CH) was partially digested with restriction endonucleases Eco RI and Bam Hi. The DNA fragments produced were religatd with T4-DNA ligase. The resulting DNA molecules were used to transform Escherichia coli C600. A number of deleted transposition-defective mutants and deleteted mutants which were still able to translocate were isolated. The results of complementation test show that the transposition function of deleted transportation defectives mutants of Tn233 (CH) can be complemented by deleted mutants still capable of transposition.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 04, 1986
- Accession Number
- ADA166299
Entities
People
- Baofu Ni
- Chen Shiyi
- Mengmin Hong
- Zongyang Wang
Organizations
- National Air and Space Intelligence Center