Characterization of Antibody-Dependent Killing of Trypanosomes by Macrophages.
Abstract
In the presence of specific antibody, murine peritoneal macrophages and macrophage-like cell lines bind Trypanosoma rhodesiense parasites in vitro. There is a high correlation between the abililty of these products to neutralize trypanosome infection in vivo and mediate binding of trypanosomes to macrophages in vitro and a high coincidence between function in the macrophage-binding assay and the ability of monoclonal antibody to bind trypanosomes in an indirect immunofluorescence assay. Trypanosomes do not actively penetrate macrophages, but instead are phagocytized by the macrophages. The C3 component of complement is an important co-factor in IgG mediated binding and uptake of trypanosomes by macrophages. There is a partial genetic regulation of this phenomenon. Normal peritoneal macrophages from C57BL/6, BALB/c, and C3H/HeJ mice do not differ in their trypanosome-bnding ability. However, 10 days post-infection, cells from genetically susceptible, BALB/c mice bound more trypanosomes than did those from infected genetically resistant, C57BL/6 mice. Keywords: Immunoglobulins; Complement.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1984
- Accession Number
- ADA167338
Entities
People
- David L. Rosenstreich
- Hellen C. Greenblatt
Organizations
- Albert Einstein College of Medicine