Liposomal-Encapsulated Stroma-Free Hemoglobin as a Potential Blood Substitute.
Abstract
We have made considerable progress in optimizing preparation of NEOHEMOCYTES, NHC. At this time we can prepare NHC suspensions, suitable for infusion, that are equivalent in tissue delivery properties to either a 17.3g% Stroma Free Hemoglobin solution or to normal blood having a hematocrit 0.20. We know that the half-time for retention of NHC in the circulation increases dramatically as the amount transfused increases. Further, we know that circulation half-time is very sensitive to changes in the membrane composition and, to somewhat less extent, to their diameter. One would prefer that circulation half-time be essentially the same for transfusions of either 10% or 90% of blood volume. To reach this goal we are preparing several 'masking lipids'. These synthetic phospholipids with inert carbohydrate head groups, are designed to minimize or prevent interaction or binding of NHC to tissues, and reduce the likelyhood of reticuloendotheial blockaid. The prospects for NHC look very good Potential antigenisity and undesirable effects on clotting time are potential problem areas that will require increasing attention. Keywords: Blood substitutes, Encapsulation methodology.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 31, 1982
- Accession Number
- ADA168129
Entities
People
- C. A. Hunt
Organizations
- University of California, San Francisco