Application of New Technologies to Radiation Biodosimetry in Mammalian Systems.
Abstract
Monoclear cells from peripheral blood are presently the best candidates for a space radiation biodosimeter for man because they are easily obtained, and are among the most radiosensitive cells in the body. They are, however, members of a highly heterogeneous population of cell subtypes potentially differing in radiosensitivities. To study the cell subpopulation holding the greatest potential for use as a biodosimeter, the cell types must be uniquely identified, and/or separated so that subtle effects on radiosensitive cells are not masked by non-effects on relatively radioresistant cells present in a mixed population. Flow cytometry and centrifugal elutriation are proving to be useful for identifying and separating individual subpopulations from heterogeneous mixtures of cells. Flow cytometry uses fluorescent antibodies to label and sort the cells of interest, while centrifugal elutriation utilizes counterbalanced centrifugal and fluid flow forces to separate cells by size. In combination with two analytical procedures (premature chromosome condensation (PCC) and alkaline elution), these two techniques have been applied to study radiation effects on mononuclear cells from mouse peripheral blood irradiated in vivo, and cell cycle phase specific repair of single-strand breaks in cellular deoxyribonucleic acid (DNA) of the Chinese hamster ovary (CHO) fibroblast cell line irradiated in vitro. Whole-body irradiation of mice produced significant decreases in total white blood cell count following as little as 100 rads. The strongest response was in the lymphocyte compartment. Radiation-induced DNA and chromosome damage in CHO fibroblasts was also studied.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1986
- Accession Number
- ADA168176
Entities
People
- Edith M. Lord
- Peter C. Keng
- Robert M. Sutherland
Organizations
- University of Rochester