A Thick-Sectioning Technique for Preservation of Bone-Metal Interfaces,

Abstract

A technique is described for preparing 100-150 micrometer thick-sections of bone plus metallic implant that has been embedded in polymethyl methacrylate (PPM). Sectioning is accomplished by using a low speed saw. PMM is removed using a xylene and eosin wash (80:20 v:v) and sections are fixed on glass slides using Eukitt's mounting medium. Bone-metallic specimens prepared in this manner allow for optimal visualization of the bone implant interface. Moreover, microscopic assessment of attendent soft and hard tissues for histomorphometry is facilitated.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1986
Accession Number
ADA168525

Entities

People

  • James Mizgala
  • Jeffrey O. Hollinger
  • John P. Schmitz

Organizations

  • United States Army Institute of Surgical Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Boundaries
  • Composite Materials
  • Embedding
  • Laboratory Animals
  • Materials
  • Metals
  • Micrometers
  • Photographs
  • Polymethyl Methacrylate
  • Research Facilities
  • Security
  • Visualizations

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Systems Analysis and Design
  • Thin Film Deposition Science.