An Investigation into the Effects of Peptide Neurotransmitters and Intracellular Second Messengers in Rat Central Neurons in Culture.

Abstract

Significant progress has been made in several areas of our research proposal. We have developed a Ca-imaging system which is now capable of resolving subcellular Ca changes on the order of seconds. This Ca-imaging system has been used to study neurotransmitter actions in culture diencephalic and cerebellar neurons. Both classical (GABA) and modulatory (thyroid hormone) neurotransmitters were found to affect cellular Ca(2+) levels in these cells without depolarizing resting potential. We have also continued studies of second messenger action in identifiable molluscan neurons. The concentration of endogenous cyclic AMP in single cells was measured, and under stimulated conditions the levels were sufficient to suggest activation of cyclic AMP-induced membrane conductances.

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Document Details

Document Type
Technical Report
Publication Date
May 21, 1986
Accession Number
ADA170020

Entities

People

  • John A. Connor

Tags

Communities of Interest

  • Biomedical
  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Amines
  • Birds
  • Blood
  • Brain
  • Cells
  • Cellular Structures
  • Culture Techniques
  • Embryos
  • High Resolution
  • Medical Personnel
  • Membrane Potentials
  • Nerves
  • Neurons
  • Neurosciences
  • Neurotransmitter Agents
  • Peptides
  • Thyroid Hormones

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Neuroscience