Production and Purification of Anthrax Toxin

Abstract

The three protein components of anthrax toxin can be safely produced and separated from each other using newly developed conditions for growth of Bacillus anthracis and recovery of culture supernatant protein. Growth of the avirulent Sterne strain in a 50-liter fermentor yields up to 500 milligram of the protective antigen component. Detailed analysis showed that this protein has a small degree of heterogeneity caused by proteolytic nicking and by a second unknown mechanism that produces species differing slightly in charge.

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Document Details

Document Type
Technical Report
Publication Date
May 20, 1986
Accession Number
ADA170131

Entities

People

  • Stephen H. Leppla

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Amino Acids
  • Animals
  • Bacteria
  • Biomedical And Dental Materials
  • Cell Line
  • Cells
  • Chromatography
  • Infection
  • Liquid Chromatography
  • Medical Personnel
  • Nucleotides
  • Peptides
  • Polymeric Films
  • Public Health
  • Resins
  • Rodents

Fields of Study

  • Biology

Readers

  • Microbial Pathology