Correlation of Mutagenic, Carcinogenic and Cocarcinogenic Effects of Chemical Substances. Granuloma Pouch Assay
Abstract
The research project is concerned with the detection of premalignant and malignant cells induced in vivo in a novel assay system the granuloma pouch assay. Cells exposed to carcinogens in vivo can be studied for DNA damage, chromosomal aberrations, specific locus mutations and cell transformations. Various assays were developed to investigate the growth characteristics of normal, carcinogen-exposed and transformed granuloma pouch cells. These included: primary cloning efficiencies under optimal growth conditions, growth in serum deficient and calcium depleted media, growth in soft agar, growth in athymic mice and rats, growth with cocultured normal cells, growth under influence of growth factors. Phenotypic alterations investigated were the DNA- dispersion in individual cells and the appearance of calcium binding proteins. Furthermore the genotoxic/cytotoxic activity of asbestos fibers was investigated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 26, 1985
- Accession Number
- ADA170862
Entities
People
- Gerhard Zbinden
- Peter Maier