Development of an Immunoassay for Bacterial Endotoxins
Abstract
This report describes (1) progress in establishing and validating an immunoassay for lipopolysaccharides; and (2) steps taken to begin to characterize the antigenic site of LPS which is involved in the assay. In step 1 of the assay polystyrene tubes are coated with Re595-LPS, washed with bovine serum albumin solution, and dried. In step 2 the sample to be assayed is mixed with I(125)-labelled immunopurified anti-(Re595-LPS) IgG and allowed to form soluble antigen-antibody complexes. The mixture is transferred to an LPS coated tube, the uncomplexed I(125)-IgG binds to the tube, and the soluble LPS-antibody complexes are washed away. Finally in step 3 the LPS coated tubes with I(125)- IgG bound to them are conducted to determine the I(125) content and thus the amount of bound IgG. These data are then used to prepare a standard curve or to calculate the concentration of LPS in a sample.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 12, 1983
- Accession Number
- ADA171375
Entities
People
- Peter S. Tobias
- Richard J. Ulevitch
Organizations
- Scripps Research