Study of the Pathogenicity of Aeromonas hydrophila for Man
Abstract
Five Aeromonas hydrophila strains were selected from among more than 50 candidate strains collected from Thailand, Western Australia, Canada, and the U.S. They were selected because of possession of well characterized virulence properties or because they were clearly implicated in cases of human diarrheal illness. The strains were tested biochemically and toxin production and hemagglutination patterns were characterized. Each strain was then fed to groups of 3 or 4 adult volunteers. The strains were shed by the volunteers only sporadically and, except for two volunteers transiently passing unformed stools, sustained illness did not develop. This study failed to show a relationship between virulence properties as now understood and pathogenicity of Aeromonas for humans. In studies looking at the etiology of diarrhea among groups of U.S. adult students in Mexico we found that non-EPEC serotypes of enteroadherent E. coli (EAEC) were isolated from 12% of diarrhea cases overall and from 30% of the patients from whom no other agents were identified. Six of 13 students from whom paired sera were collected showed a 4 fold or greater rise in antibodies in serum. Two stains of EAEC were selected for study in volunteers. In doses of 700 million and 1 x 10 to the 10th power viable cells, strain JM 221 produced a diarrheal illness in 4 of 8 subjects while 1 of 8 fed strain 189 developed a diarrheal illness. Test strains were recovered from stool throughout the study indicating intraluminal or intraintestinal replication of the test strains.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 15, 1984
- Accession Number
- ADA171379
Entities
People
- Herbert L. Dupont
Organizations
- University of Texas Health Science Center at Houston