Development of Serologic Assays for the Diagnosis of New World Leishmaniasis.
Abstract
Two-hundred eighty-nine monoclonal antibodies have been generated to surface and intracellular antigens of various isolates of New World Leishmania. Efforts have focused upon using these monoclonals to detect antigenic difference between Leishmania mexicana mexicana and Leishmania braziliensis panamanensis. Indirect immunofluorescent antibody assays have been used to demonstrate qualitatige differences; enzyme linked immunosorbent assays have been used to demonstate quantitative differences. We appear to have at least 6 monoclonal antibodies which can distinguish between air-dried promastigotes of the two species. Additional monoclonals react with both species but the intensity of reactivity is often significantly different. In fact, we now have some degree of confidence in our ability to differentiate L.m. mexicana from L.b. panamanensis on the basis of the quantitative differences.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 31, 1984
- Accession Number
- ADA171387
Entities
People
- Ronald L. Anthony
Organizations
- University of Maryland, Baltimore