Microwave-Induced Cataracts of the Eye Lens. Strategies for Modelling and Prevention In Vitro and In Vivo.
Abstract
We have developed a unique system for study of the development of microwave induced cortical cataracts of the eye lens, using as a model system the rat lens incubated in circulating tissue culture medium at controlled temperature, while being exposed to microwave irradiation. Although in unirradiated controls no cataracts occur, we have used levels of microwave irradiation at which characteristic cataractous opacities with associated globular degeneration occur. We plan to continue our studies of microwaves, using the following techniques: 1) scanning and 2) transmission electron microscopy, 3) high voltage electron microscopy, (a powerful technique for detecting alterations in cytoskeleton structure), 4) cryological electron-stimulated detection of x-rays (EDX) (a technique for examining the ionic composition of fresh tissue), and (5) biochemistry. In these studies we hope to elucidate the mechanism of the previously unreported unique effects of high energy pulsed microwave, which appear to be related to thermoacoutic transduction. In the studies to date we have established several caytaractogenic does but not looked at the minimum effective dose, and have noted several features of the irradiated lens which are apparently related to the power temperature and duration of the irradiation and to the mode of delivery (constant amplitude (CW) or high energy pulsed (Pu)).
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1983
- Accession Number
- ADA171458
Entities
People
- John R. Trevithick
Organizations
- Western University