The Etiology and Pathogenesis of Viral Gastroenteritis.
Abstract
The purpose of this project is to identify, cultivate, and characterize etiologic agents of viral gastroenteritis of man, and to study the epidemiology and pathogenesis of infection in order to provide information necessary to attain the ultimate goals of prevention and cure of this common syndrome. Progress achieved during the present contract year can be summarized as follows. A monoclonal antibody reagent has been developed that is directed against the group specific antigen shared by mammalian rotaviruses; this reagent has then been used to develop two novel rapid diagnostic tests for rotavirus in human stools. These tests provide shortened performance times as well as increased sensitivity and specificity compared to the currently commercially available assay. We are now building upon this experience gained with rotavirus to develop monoclonal antibodies against Norwalk virus, which is to data noncultivatable and fails to produce illness in animal model systems. Approaches are being taken to circumvent logistical problems that have developed in the preparation of monoclonal antibodies to stool derived Norwalk virus. When produced, these antibodies will permit analysis of the bilogy of Norwalk virus, provide a potential diagnostic probe for a possible group-specific antigen common to the noncytopathic Norwalk-like group, and permit recognition of these viruses in nature. In addition, a series of experiments designed to cultivate Norwalk virus in vitro have been undertaken.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 31, 1983
- Accession Number
- ADA171858
Entities
People
- Neil R. Blacklow
Organizations
- University of Massachusetts Medical School