Oligonucleotide Fingerprinting of Dengue Virus RNA to Support Ongoing Epidemiological Investigations.
Abstract
An information system for analyzing newly determined dengue virus fingerprints in the context of an on line data base of previously determined fingerprints is being developed and tested. We are digitizing the original autoradiograms and then analysing the resulting digital image to produce files of features that allow rapid reconstruction of a good facsimile of the original spot pattern on a suitable graphics device. These feature files allow sufficiently efficient storage of the information that we will be to maintain a large data base on line in a dedicated computer. We also have developed preliminary software to register the images so that rapid judgements can be made about the number of spots that have equivalent mobilities on any two images. Software is also available to use data for many such binary comparisons to perform cluster analysis to determine hierarchical relationships among the various viral strains. Considerable progress has been made in most areas of the project. The next step is to integrate the various pieces into a unified system. Keywords: Segmentation, and Image rectification.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 13, 1984
- Accession Number
- ADA172382
Entities
People
- George E. Fox
Organizations
- University of Houston