Cellular Effects of Perfluorinated Fatty Acids.

Abstract

The compound perfluoro-n-decanoic acid (PFDA) was exposed to three tissue culture cell lines, PTK2 (kidney), BRL (buffalo rat liver) and K9 (clone 9, rat liver) in concentrations up to 200 micrograms/ml for 24 hr. time periods. The technique of fluorescence recovery after photobleaching (FRAP) was used ot examine cell membrane fluidity, specifically with respect to the membrane lipids. Results demonstrated that PFDA affects the fluidity of both liver lines but not the kidney line. Furthermore, differential sensitivity was observed between the responsiveness of the two liver lines. (BRL was more sensitive than K9.) The increased PFDA-induced membrane fluidity was transient: recovery occured by 48 hr following removal of the PFDA. The FRAP technique appears useful in screening agents for membrane effects as well as elucidating mechanisms of agent action.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1985
Accession Number
ADA173061

Entities

People

  • Michael W. Berns

Organizations

  • University of California, Irvine

Tags

DTIC Thesaurus Topics

  • Cell Line
  • Cell Membrane
  • Cells
  • Electron Microscopes
  • Electron Microscopy
  • Fatty Acids
  • Lipids
  • Measurement
  • Membrane Fluidity
  • Membrane Lipids
  • Microscopes
  • Scanning Electron Microscopes
  • Scanning Electron Microscopy
  • Security
  • Sensitivity
  • Tissue Culture
  • Tissues

Fields of Study

  • Biology

Readers

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  • Toxicology/Environmental Toxicology