Potential for Cross-Reactive Protection Using Peptides and Adjuvants or Carrier Molecules

Abstract

We have reported that an influenza virus-specific polypeptide produced in E.coli induced influenza virus-subtype-specific memory and secondary H-2 restricted CTL responses in mice. The c13 protein is a hybrid protein of the first 81 amino acids of the NS1 viral non-structural protein and the HA2 subunit of the viral hemagglutinin. We now report results which show that target cells exposed to c13 protein are lysed by virus-immune CTL in a subtype-specific H-2 restricted manner. This suggests that this protein interacts with target cell membranes and is presented on the cell membrane in proper confirmation with H-2 antigens of recognization by the influenza virus-specific CTL. Further, we show that immunization with this molecule results in the induction of virus-specific CTL, which are protective, and this peptide induce CTL without the need for adjuvants. Keywords: Cytotoxic T Lymphocytes, Lymphokines, Epitopes.

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Document Details

Document Type
Technical Report
Publication Date
Oct 07, 1986
Accession Number
ADA173164

Entities

People

  • Francis A. Ennis

Organizations

  • University of Massachusetts Medical School

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antigens
  • Cell Membrane
  • Cells
  • Freund'S Adjuvant
  • Identification
  • Infection
  • Influenza
  • Lymphatic System
  • Lymphocytes
  • Military Research
  • Molecules
  • Proteins
  • T Lymphocytes
  • Tissues
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Virology (or Medical Virology).