Purification of N-Acetylgalactosaminidase by Isoelectric Focusing.
Abstract
N-acetylgalactosaminidases, enzymes which convert type A human red blood cells to type O, have been purified from human placenta and from culture medium of Clostridium paraputrificum. Their properties have been studied and their ability to remove type A determinants has been quantitated by radioimmunoassay. This is the final technical report on Contract N00014-83-K-0339. Type O blood can be given to all recipients and the availability of such cells would simplify inventory and cross matching during emergencies. Chemically the A antigens differ from the O antigens in having an additional terminal sugar residue, an alpha linked N-acetyl- galactosamine. Several enzymes have been studied which remove this residue and convert A substance to O substance. These enzymes, alpha-N-acetyl-galactosaminidases, are referred to as Azymes in this report.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1986
- Accession Number
- ADA173478
Entities
People
- Ray K. Brown
Organizations
- Wayne State University