Choline Uptake by Glomerular Synapses Isolated from Bovine Cerebellar Vermis.

Abstract

Tritium Choline uptake was investigated in a highly enriched preparation of glomerular particles isolated from bovie cerebellar vermis. Kinetic analysis indicates that a high affinity uptake sytstem with a relatively low maximum uptake velocity is present. At a substrate concentration of .0000001 M, Tritium choline uptake was shown to be sodium-dependent, hemicholinium-sensitive and nonreactive to increasing concentrations of unlabeled choline. These findings support the presence of a high affinity choline uptake system in cerebellar glomeruli and would seem to be consistent with the suggestion that a small proportion of mossy fibers are cholinergic in this brain region.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1986
Accession Number
ADA174605

Entities

People

  • D. M. Terrian
  • E. L. Noisin
  • W. E. Thomas

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Aerospace Medicine
  • Air Force
  • Air Force Facilities
  • Brain
  • Cells
  • Cerebellum
  • Contracts
  • Glomeruli
  • Incubation
  • Materials
  • Nervous System
  • Neurons
  • Particles
  • Rodents
  • Scientific Research
  • Subcellular Fractions

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Neuroscience