Clostridium perfringens Spore-Lytic Enzymes.

Abstract

Enzymes which cause the germination of Clostridium perfringens spores were isolated and characterized. Two were investigated in detail. One was extracted from spores of the same organism. The second was excreted by vegetative cells of C. perfringens. The molecular weight and various characteristics of each was determined. The second enzyme is an amidase. The mode of action of the first is unknown but it could only be obtained by reagents known to disrupt the spore coat. A fluorescence assay for determining the hydrolysis product of these enzymes which will help future work in this area was developed. Results indicated the presence of a third spore-lytic enzyme associated with the wall of the organism. It's role and function is unknown.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 10, 1983
Accession Number
ADA174824

Entities

People

  • Ronald Labbe

Organizations

  • University of Massachusetts Amherst

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Cells
  • Cellular Structures
  • Clostridium
  • Contracts
  • Enzyme Inhibitors
  • Enzymes
  • Gel Electrophoresis
  • Germination
  • Hydrolysis
  • Medical Personnel
  • Molecular Weight
  • Scientists
  • Spores

Readers

  • Analytical Chemistry
  • Microbial Pathology