A Comparative Study Regarding the Association of Alpha-2U Globulin with the Nephrotoxic Mechanism of Certain Petroleum-Based Air Force Fuels

Abstract

Fischer 344 male rats have a dose and time dependent proximal tubular degeneration induced by certain petroleum-based fuels. This degeneration may be associated with a low molecular weight alpha globulin (termed alpha-2U globulin) . A new method was developed to obtain monospecific immunologic reagents for alpha-2U globulin using diafiltration, anion-exchange and hydroxylapatite chromatography. Rocket immunoelectrophoretic and isoelectric focusing techniques were developed to quantitatively and qualitatively assess changes in alpha-2U globulin after experimental exposure to hydrocarbon compounds. Keywords: Nephrotoxicity; Alpha-2u globulin; Rat urinary protein; Petroleum products.

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Document Details

Document Type
Technical Report
Publication Date
Oct 31, 1986
Accession Number
ADA175104

Entities

People

  • Thomas E. Eurell

Organizations

  • University of Illinois Urbana–Champaign

Tags

Communities of Interest

  • Weapons Technologies

DTIC Thesaurus Topics

  • Air Force
  • Alpha Globulin
  • Animals
  • Chemistry
  • Complex Mixtures
  • Ecotoxicology
  • Environmental Pollutants
  • Gamma Globulin
  • Globulins
  • Immune Serums
  • Lagomorphs
  • Medical Personnel
  • Molecular Weight
  • Proteins
  • Rodents
  • Toxicology
  • Universities

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Immunology
  • Molecular and Cellular Biochemistry