Dermal Influence on Epidermal Resurfacing during the Repair of Split Thickness Wounds.
Abstract
The purpose of this study is to evaluate dermal-epidermal interrelationships during wound repair by assessing the effect on epidermal wound healing of factors derived from platelets. Two methods were used to approach the problem: 1) a skin explant culture model in which small sections of partial thickness pig skin (explants) were grown in medium containing fetal bovine serum and/or a platelet homogenate, and epidermal cell outgrowth form the explant was measured; 2) a wound healing animal model in which partial thickness excisional wounds made on the backs of young domestic pigs were treated with platelet preparations then evaluated for epidermal resurfacing and dermal collagen biosynthesis. Our results are as follows: (1) in explant cultures: (a) Platelet Homogenate Fraction initiated cell outgrowth without serum; (b) Platelet Homogenate Fraction stimulated the rate of outgrowth; (c) The stimulatory factors were stable to heating at 100 C for 2 min; (d) Commercial PDGF without serum did not support explants. With serum it had a slight stimulatory effect; and (e) Platelet Homogenate Fraction together with serum was able to produce an inhibition of explant viability and outgrowth. (2) In wound healing: (a) Topical application of Platelet Homogenate (5 min/day for 7 days) did not affect epidermal or dermal healing; and (b) Topical application of Platelet Homogenate with Occlusion (for 2 hr or 6 hr) enhanced epidermal wound healing compared to Air Exposed wounds, but was not better than Vehicle with Occlusion.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 15, 1984
- Accession Number
- ADA175117
Entities
People
- Particia Hebda
- William H. Eaglestein
Organizations
- University of Pittsburgh