Host Defense against Opportunist Microorganisms Following Trauma.
Abstract
The temporal occurrence of multiple immunologic alterations resulting from thermal injury was investigated in guinea pigs with scald burns of 30% total body surface. Groups of 2-4 burned and sham-treated animals were sacrificed at 1-3 day intervals during 3 weeks postburn. C3 concentration, total and alternative complement pathway mediated C3 fixation on Pseudomonas aeruginosa, proportion of native C3 to C3 cleavage fragments, and prostaglandin (PG) E2 level were measured in serum or plasma. Bactericidal activity of peripheral polymorphonuclear leukocytes (PMN) against P. aeruginosa, proliferative responses of splenic lymphocytes to phytohemagglutinin and concanavalin A, and clearance of radiolabeled Pl. aeruginosa by the reticuloendothelial system were also determined. Complement consumption with concomitant reduction in C3 concentration and bacterial C3 fixation, and elevation of PGE2 occurred within 3-6 h postburn. These alterations were accompanied by reduction in intrinsic PMN bactericidal activity, suppression of PMN bactericidal activity by serum, and a minor decrease of blood clearance of P. aeruginosa. Complement and PMN dysfunction returned to normal by the end of the first week postburn. A clear temporal separation in the occurrence of depression in lymphoproliferative response was evident, since this alteration was not observed until 4 days postburn was maximal during 7-9 days postburn.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 27, 1984
- Accession Number
- ADA175119
Entities
People
- Ann B. Bjornson
- H. S. Bjornson
- Josef E. Fischer