An Investigation of the Memory Response of the Local Immune System to Shigella Antigens.
Abstract
The present studies center on optimizing the mucosal humoral immune response to Shigella flexneri. Using an isolated ileal loop model system in rabbits, the present work demonstrates that a hyperstimulation of the primary local IgA response can be elicited by a combination of parenteral and oral immunization. The rapid production of secretory IgA against S. flexneri cound be especially useful in combating sudden outbreaks of enteric disease. Cell culture techniques are described which will be used to learn how to best stimulate cells that are committed to synthesize IgA anti-shigella lipopolysaccharide. Lastly, the role played by mucosal humoral immunity in host defense against dysentery is still unclear. We are in the process of developing both in vivo and in vitro model systems which will allow us to document the role of the anti-shigella mucosal immune response in preventing adherence, invasion and ulceration. Such assay systems are critical to predicting the utility of mucosal vaccines to a variety of enteropathogens.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 1985
- Accession Number
- ADA175215
Entities
People
- David F. Keren
Organizations
- University of Michigan