Fellowship Program for the Study of Recombinant DNA Techniques in Biochemistry.
Abstract
This fellowship was used to support two graduate students whose projects focused on different aspects of the biology of bacteriophage T4: 1, RNa processing; 2, the shut-off of host transcription during T4 infection; and 3, inhibition of proteolysis. In the first project, two T4 introns were identified and the products of the autocatalytic splicing reaction were characterized. In an attempt to determine the phage genes involved in the shut-off of host transcription, multiple mutant phage were constructed. These phage incorporate mutations in all genes known to affect Host DNA structure and transcription, but were still able to shut off host functions with nearly normal kinetics. Finally, bacteriophage T4 has been developed as a vector for expressing foreign proteins while simultaneously stabilizing certain proteins that are normally rapidly degraded in E. coli.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 28, 1986
- Accession Number
- ADA175639
Entities
People
- Ann Zeeh
- Henry Tedeschi
- Jonatha Gott
- Richard Zitomer
Organizations
- State University of New York at Albany