Wound Coverage by Cultured Skin Cells.
Abstract
The major purpose of our work is to refine the technology for growth of human epidermal cells to achieve more rapid growth in vitro and easier handling of tissue cultured materials in clinics. It is also to evaluate the possibilities for the use of allogeneic epidermal cells grown in vitro for wound healing. For the studies in vitro we used various tissue culture techniques. Quantitative data were obtained by cell counts and incorporation of radioactively labeled precursors, and qualitative data by light and electron microscopy. Studies in vivo were performed on experimental animals - swine. Careful visual observations were documented histologically and by photography, They were supplemented by immunological approaches such as immuno-rosetting and studies of lymphocytes (MLC). We have defined conditions for growth of epidermal cells on colloagen matrices. Similarly, we have optimized conditions for preparing dermal grafts which are composed of collagen sponges (collagen type I) and fibroblasts. It was shown that such materials are suitable for transplantation and that their handling in clinical settings is acceptable. We have also shown that healing by epidermal cells without a collagen substrate. Moreover, the combination of dermal grafts and epidermal grafts resulted in healing of some deep, chronic wounds. For the studies of allogeneic grafts, major emphasis was placed on development of techniques for rapid detection of major histocompatibility antigens (SLA) and identification of skin specific antigens. Immuno-rosetting with protein A coated erthrocytes proved a sensitive and specific method. Skin specific antigens were revealed on keratinocytes using sera obtained by skin transplantation between SLA matched animals.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 09, 1985
- Accession Number
- ADA175894
Entities
People
- Ellen M. Duffy
- Magdalena Eisinger
Organizations
- Memorial Sloan Kettering Cancer Center