Studies of RBC Preservation in Vivo in a Rabbit Model.
Abstract
During blood storage at 4C, a fraction of RBC become nonviable. The larger this fraction, the poorer the quality of RBC preservation. Unfortunately, the fraction of viable RBC in stored blood can be measured only by survival studies in vivo. Human studies are difficult due to limited numbers of willing subjects. Thus, we have investigated whether the data obtained in this model are applicable to the human system. In our studies with the rabbit model we have paid attention to some current issues of interest, namely to the comparison of the RBC viability measurements preserved in ADSOL, CPDA-1 and CPD. Furthermore, we have evaluated the viability of Tc-99m as an RBC label. Other studies done in this period of time were related to our attempt to elucidate whether RBC-bound C3 plays a role in destruction of preserved damaged RBC. Our data also showed that more radioactivity, derived from preserved RBC was found in the liver than in the spleen 24 hours after transfusion, suggesting that complement might be involved in eliminating the damaged preserved RBC.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 10, 1985
- Accession Number
- ADA175964
Entities
People
- Irma O. Szymanski
Organizations
- University of Massachusetts