Structure and Functional Studies of DEN-2 Virus Genome.
Abstract
The progress made in the project can be grouped in three parts focussing on the central goal of identifying and characterizing the gene for Dengue virus glycoprotein antigen E. Cloning of complementary DNA (cDNA) copy of Dengue viral RNA into E. coli HB101/pBR322 host/vector system and characterization of a clone that contained a 400 bp insert complementary to Dengue RNA will be described. Construction of a cDNA library using Dengue RNA as temperate and a random mixture of short oligodeoxy nucleotides (6-8 nucleotides long) as primers for reverse transcriptase will be described. The double-stranded cDNA molecules that were synthesized in this approach were cloned using M13mp8 vecotr and E. coli JM103 as host. Several positive recombinant bacteriophage plaques were picked based on the insertional inactivation of Beta-galactosidase marker gene present in the vector. Details regarding further screening of these positive plaques by size analysis of the recombinant DNA and by Southern hybridization will be given. DNA sequence analysis of some of these clones will be described.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1983
- Accession Number
- ADA176500
Entities
People
- Radha K. Padmanabhan
Organizations
- University of Kansas Medical Center