Effects of Phenyldichlorarsine on Macrophages and Endothelial Cells: A Preliminary Ultrastructural Study.

Abstract

The cellular mechanisms involved in arsenical-induced vesication are discussed. To approach this problem, tissue culture studies were undertaken to correlate morphological alterations with previously reported changes in cell growth that are induced by phenyldichlorarsine (PDA), a monosubstituted trivalent arsenical. Changes in the morphology of human capillary endothelial cell cultures and RAW 264.7, an established line of mouse macrophage cells, were monitored by scanning and transmission electron microscopy. Study results indicate that over a broad range of concentrations, PDA induces morphological changes in both mouse macrophages and human endothelial cells. The observed morphological alterations indicate that, even at low concentration, PDA can affect cellular processes. Keywords: RAW mouse macrophage, Human vascular endothelium, Cell culture, Vacuole; Cell membrane.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 1986
Accession Number
ADA176549

Entities

People

  • John W. Harbell
  • Michael P. Sarras Jr.
  • Steven T. Schuschereba

Organizations

  • Letterman Army Hospital

Tags

DTIC Thesaurus Topics

  • Biological Sciences
  • Blood
  • Cardiovascular System
  • Cell Membrane
  • Cell Membrane Structures
  • Cells
  • Cellular Structures
  • Culture Techniques
  • Cultured Cells
  • Electron Microscopes
  • Electron Microscopy
  • Endoplasmic Reticulum
  • Intercellular Junctions
  • Microvessels
  • Military Research
  • Tissue Culture
  • Tissues

Readers

  • Cardiovascular Physiology
  • Geochemistry
  • Oncology (Cancer Research).

Technology Areas

  • Microelectronics